Region of interest: Tracking power should not be wasted on regions of the image that are outside of the arena, which can also confuse the tracker.Tracking type: Dark flies on a light background, or light flies on a dark background.
The tracking wizard sets the following parameters, in order: Many users will still need to perform detailed parameter-setting as described below, but the Tracking Wizard can provide guided initialization for that process. For simple and easy arena configurations, the Tracking Wizard may be sufficient to provide high-quality tracking. The Tracking Wizard helps set the most common necessary parameters for tracking. mat file for use with the Fi圎rrors Matlab GUI, the BehavioralMicroarray Matlab Toolbox, or other Matlab code: Ctrax File->Export as MAT-file. When tracking is complete, resolve the 180-degree orientationĪmbiguity: Ctrax Track->Choose Orientations.Begin tracking: Ctrax Track->Start Tracking.Set the tracking parameters: Ctrax Settings->Tracking.Set the background-subtraction parameters: Ctrax Settings->Background Subtraction.Compute the background model: Ctrax Settings->Background.More settings are available through this method than through the Tracking Wizard, and such detailed tracking configuration may or may not be necessary. Run the Tracking Wizard: Ctrax Track->Tracking Wizard.Alter the tracking settings to match your video, by one of the following two methods:.To track a video, perform the following steps: The main panel shows a frame of the video. Screenshot of main Ctrax window (right) and zoomĬtrax will then bring up the main Ctrax window, shown at right. A backup copy of the previous tracking results is created in the same directory.
Qbserve tracking video movie#
If the movie has previously been tracked, Ctrax will read in the parameters and trajectories, and tracking can be either resumed or restarted from the beginning. This annotation file will contain the flies' trajectories, as well as all parameters used during tracking. The tracking results will be saved in the same location as the movie file, with the extension. See Input Video Formats for information on the types of video Ctrax currently supports. The first order of business after starting Ctrax is to open a video to be processed by selecting File->Open.
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